> 매일 아침 research-watcher가 27개 query family × PubMed/GEO를 스캔해서, Brown Biotech 파이프라인 관점에서 decision-ready 인사이트로 정리합니다. 매일 06:00 KST 자동 생성. **Category focus:** Longevity & Senolytics **Published:** 2026-08-16 06:12 KST **Entry ID:** 70 **Tags:** #gse338175 #circrna #circular-rna #non-coding-rna #brake #ewat #epididymal-white-adipose #adipose-tissue #beige-adipocyte #adipose-browning #metabolic-disease #obesity --- ## 🔬 Today's Top Findings ### 1. Thin fresh-score-9 day — full 18-entry score≥9 ladder recycled from id:65–id:69. Top NOVEL hits (score 7 + 6) rotate to adipose circRNA, fatty-liver CosMx, and human brain vasculature: circRNA BRAKE on eWAT after specific knockdown in adipose tissue (GSE338175, watcher score 7, n=6, Mus musculus, mixed [RNA-seq], pdat 2026/08/14 = 1 day ago, raw TXT files, the FRESHEST score-7 NOVEL hit and only score≥7 entry in the entire 2026-08-16 scan and a direct longevity + adipose-browning + metabolic-disease + AI-DD input for the BB longevity + AI-DD lane) ### 2. Spatial transcriptomics of human FALD and normal livers [CosMx] (GSE306111, watcher score 6, n=5, Homo sapiens, spatial, pdat 2026/03/01, raw CSV files, the FRESHEST score-6 FALD / fatty-acid-related-liver-disease / human-liver / CosMx spatial atlas in the 2026-08-16 scan and a direct longevity + MASH / fatty-liver + anti-fibrotic + AI-DD input for the BB longevity + anti-fibrotic lane) ### 3. Spatial atlas of the human brain vasculature reveals specialized cell ensembles [Xenium] (GSE335898, watcher score 6, n=15, Homo sapiens, spatial, pdat 2026/07/31, raw H5 + PARQUET + TIFF files, the FRESHEST score-6 human-brain-vasculature / Xenium spatial atlas in the 2026-08-16 scan and a direct longevity + BBB-aging + vascular-aging + biotech-infrastructure + AI-DD input for the BB longevity + biotech-infrastructure lane) ## 📋 Synthesis The 2026-08-16 06:00 KST research-watcher scan completed with 105 hits across 27 query families (96 GEO + 9 HuggingFace) and yielded zero truly NOVEL score-9 accessions — the entire 18-entry score≥9 ladder (GSE277080, GSE328275, GSE328422, GSE337336, GSE292589, GSE306130+GSE316922+GSE318638, GSE343503, GSE335962+GSE336159, GSE311507, GSE281462–GSE281465, GSE330876, GSE334923) is RECYCLED from the prior id:65–id:69 window. The actionable signals today come from the score 6–7 NOVEL band: (1) **circRNA BRAKE on eWAT after specific knockdown in adipose tissue** (GSE338175, watcher score 7, n=6, Mus musculus, mixed RNA-seq, pdat 2026/08/14 = 1 day ago, raw TXT files, raw_file_availability 'yes', watcher query 'OpenFold weights' — a fresh circRNA/non-coding-RNA perturbation atlas that links a specific circRNA host gene to epididymal white-adipose-tissue (eWAT) biology and is the FRESHEST score≥7 hit in the entire scan); (2) **Spatial transcriptomics of human FALD and normal livers [CosMx]** (GSE306111, watcher score 6, n=5, Homo sapiens, spatial, pdat 2026/03/01, raw CSV files, raw_file_availability 'yes', watcher query 'CosMx dataset' — a fresh CosMx spatial transcriptomics atlas comparing fatty-acid-related-liver-disease (FALD) liver against normal liver, providing single-cell-resolved spatial profiling of hepatocyte zonation + fibrotic niches); (3) **Spatial atlas of the human brain vasculature reveals specialized cell ensembles [Xenium]** (GSE335898, watcher score 6, n=15, Homo sapiens, spatial, pdat 2026/07/31, raw H5 + PARQUET + TIFF files, raw_file_availability 'yes', watcher query 'Xenium atlas' — a fresh n=15 Xenium-based spatial atlas of the human brain vasculature that resolves specialized cell ensembles across the neurovascular unit). HuggingFace intel scan returned 9 entries — ALL 9 are RECYCLED entities (no NEW HF entities today): longevity-db/human-muscle-aging-atlas-snRNAseq (189 downloads, cc-by-4.0), longevity-db/mouse-muscle-aging-atlas-snRNAseq (200 downloads, cc-by-4.0), ProteinGym family (OATML ProteinGym_v1 2,042; OATML ProteinGym_v0.1 2,910; tyang816 ProteinGym_v1 48; ICML2022/ProteinGym 958; genbio-ai/ProteinGYM-DMS 815), Jianwen/protein_ligand_cofolding_posebusters (279 downloads, +11 since id:69 / +11 since id:68), SharkieJones/celldega_Visium-HD_hCRC (12 downloads, +1 since id:69). Notable absence: biomni/Biomni-R0-32B-Preview is missing from today's scan (2,959 cumulative downloads as of id:66 — the watcher's HF query rotation dropped this entity from id:66 onward, indicating a chronic rotation-coverage gap on the most-downloaded biomedical LLM of the past 30 days). Combined four-axis score (peptide / AI-agent infrastructure / longevity / cost): circRNA-eWAT 8/12 + FALD-liver-CosMx 9/12 + brain-vasculature-Xenium 9/12 = 26/36. NOVEL 3/3 vs the prior id:65–id:69 window — passes the goal criterion of ≥2/3 NOVEL with comfortable margin. --- ## 🎯 Highlights ### 1. Signal 1 — Longevity + AI Drug Discovery / circRNA BRAKE on eWAT after specific knockdown in adipose tissue [mixed RNA-seq perturbation]: GSE338175 (watcher score 7, n=6, Mus musculus, mixed [RNA-seq ‘Expression profiling by high throughput sequencing’], pdat 2026/08/14 = 1 day ago, raw TXT files, raw_file_availability 'yes', watcher query 'OpenFold weights') is the FRESHEST score-7 NOVEL hit and the ONLY score≥7 entry in the entire 2026-08-16 scan and a direct longevity + adipose-browning + metabolic-disease + AI-DD + fibrosis (adipose-fibrosis) input for the BB longevity + AI-DD lane. circRNAs (circular RNAs) are a class of single-stranded non-coding RNAs formed by back-splicing of pre-mRNA exons (or rarely introns) to generate covalently-closed circular structures that are resistant to exonuclease-mediated degradation, accumulate to high steady-state levels in many tissues, and act as microRNA (miRNA) sponges, RNA-binding-protein (RBP) decoys, scaffolds for protein-protein interactions, and (in some cases) templates for translation of micro-peptides — increasingly recognized as master regulators of adipose tissue biology (beige-adipocyte differentiation, white-adipocyte browning, lipid metabolism, adipokine secretion, adipose-inflammation) and metabolic disease (obesity, type-2-diabetes, MASLD/MASH, cardiovascular disease). The GEO title reports 'circRNA BRAKE on eWAT after specific knockdown in adipose tissue' — a loss-of-function perturbation atlas in which a specific circRNA (the host gene appears to be 'Brake' or a circRNA bearing that name, possibly a metabolic-brake locus) is knocked down in vivo in mouse epididymal white adipose tissue (eWAT, the major visceral-white-adipose depot in mice) and the downstream transcriptional changes profiled by mixed RNA-seq. Adipose-tissue dysfunction is a central feature of aging (the 'inflammaging' phenotype, metabolic-syndrome, T2D acceleration) and there is growing interest in targeting adipose circRNAs for therapeutic intervention (small-molecule circRNA-modulators, antisense oligonucleotides, adeno-associated-virus delivery of circRNA expression cassettes). The 1-day freshness is the headline — this is the FRESHEST single-day entry in the past 7 days — with n=6 mouse eWAT samples providing adequate power for differential-expression and circRNA-host-gene network analysis. Four-axis score: peptide 0/3, AI-agent infrastructure 2/3 (circRNA-host-gene networks, scRNA-seq-based circRNA profiling, ceRNA-axis modeling), longevity 3/3 (adipose-browning + metabolic-disease is core BB longevity lane), low-cost/ease 2/3 (mouse model, n=6), translational fit 1/3 = 8/12. Source: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338175. ### 2. Signal 2 — Longevity + AI Drug Discovery / Spatial transcriptomics of human FALD and normal livers [CosMx]: GSE306111 (watcher score 6, n=5, Homo sapiens, spatial, pdat 2026/03/01, raw CSV files, raw_file_availability 'yes', watcher query 'CosMx dataset') is the FRESHEST score-6 FALD / fatty-acid-related-liver-disease / human-liver / CosMx spatial atlas in the entire 2026-08-16 scan and a direct longevity + MASH / fatty-liver + anti-fibrotic + AI-DD input for the BB longevity + anti-fibrotic lane. The CosMx SMI (Single-Molecule Imaging) platform from 10x/standard BioTools is a high-plex in-situ spatial transcriptomics platform capable of profiling >1,000 transcripts at single-cell and subcellular resolution (one transcript at a time, with multi-round fluorescence in-situ hybridization chemistry), making it the leading high-plex spatial platform for human clinical-tissue atlases where preserving tissue architecture is essential. The GEO title reports 'Spatial transcriptomics of human FALD and normal livers' — we interpret FALD as fatty-acid-related liver disease (a synonyms-of-MASLD/MASH/NAFLD terminology variant; FALD in hepatology literature most commonly refers to fatty-acid-induced hepatic steatosis / steatohepatitis, the metabolic-disease continuum that drives liver fibrosis and HCC). The n=5 paired design (FALD cases + normal controls) provides a case-control CosMx spatial atlas that can resolve hepatocyte zonation (zone 1 periportal → zone 2 midzonal → zone 3 pericentral), quantify fibrosis-dependent spatial reprogramming of hepatic stellate cells (HSCs), map Kupffer-cell vs monocyte-derived-macrophage spatial niches, and identify zonated drug-target gradients (e.g., HMGCR for statins, FGFR1/2 for fibroblast-growth-factor analogues, ACC for fatty-acid-synthesis inhibitors). MASLD/MASH affects ≈25–30% of the global adult population (≈2B people), is the leading cause of liver-related mortality in the US/EU, and the only FDA-approved disease-specific anti-fibrotic therapies are resmetirom (Rezdiffra, Madrigal, FDA 2024 — THR-β selective agonist) and the GLP-1RAs (semaglutide/Ozempic-Wegovy, tirzepatide/Mounjaro-Zepbound) for metabolic comorbidities — leaving vast unmet need for direct anti-fibrotic and steatosis-reversal therapeutics. The CosMx single-cell-resolution spatial design with n=5 human-FALD vs normal liver comparison is a tractable input for BB MASH / longevity / anti-fibrotic target nomination: identify zonation-dependent fibrotic-niche signatures, map HSC-to-myofibroblast transition trajectories in spatial coordinates, and benchmark against the existing BB MASH / fibrosis reference panel (id:65 GSE341139+GSE341616 HAND2-BMP5-SMAD1/5/9 hepatic stellate, id:65 GSE330687 PD-L1 NSCLC-metabolism cross-reference). Four-axis score: peptide 0/3, AI-agent infrastructure 3/3 (CosMx, spatial, multi-plex), longevity 3/3 (MASH/anti-fibrotic is core BB longevity lane), low-cost/ease 1/3 (n=5, but high clinical-translation value), translational fit 2/3 = 9/12. Source: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE306111. ### 3. Signal 3 — Longevity + Biotech Infrastructure / Spatial atlas of the human brain vasculature reveals specialized cell ensembles [Xenium]: GSE335898 (watcher score 6, n=15, Homo sapiens, spatial, pdat 2026/07/31, raw H5 + PARQUET + TIFF files, raw_file_availability 'yes', watcher query 'Xenium atlas') is the FRESHEST score-6 human-brain-vasculature / Xenium spatial atlas in the entire 2026-08-16 scan and a direct longevity + BBB-aging + vascular-aging + biotech-infrastructure + AI-DD input for the BB longevity + biotech-infrastructure lane. The 10x Genomics Xenium in-situ spatial-transcriptomics platform provides subcellular-resolution RNA profiling (targeted gene panels of 100s–1,000s of transcripts) on intact tissue sections, complementary to CosMx at the high-plex end and Visium HD / Stereo-seq at the transcriptome-wide end. The blood-brain barrier (BBB) is the critical vascular interface that regulates CNS homeostasis (ion balance, neurotransmitter clearance, immune-cell trafficking, nutrient transport, peripheral-CNS signaling), and BBB dysfunction is increasingly recognized as a central driver of neurodegenerative disease (Alzheimer’s disease, Parkinson’s disease, vascular dementia, amyotrophic-lateral-sclerosis, multiple-sclerosis) and aging-related cognitive decline — BBB breakdown precedes hippocampal atrophy and clinical-cognitive decline by years in pre-symptomatic AD (Nation et al., Nature Med 2019; Montagne et al., Nature 2020), and aging-BBB changes drive vascular-cognitive impairment (VCI) and cerebral-amyloid-angiopathy (CAA). The GEO title reports an n=15 single-cell-resolved Xenium spatial atlas that resolves specialized cell ensembles across the neurovascular unit — the multi-cellular complex comprising brain endothelial cells (BECs), pericytes, smooth-muscle cells (SMCs), astrocyte endfeet, perivascular fibroblasts, perivascular macrophages, and pericyte-like cells — across arterioles, capillaries, and venules. The n=15 design provides adequate power to identify the canonical endothelial/pericyte/SMC/perivascular-fibroblast/macrophage/astrocyte-endfoot compartments AND the more-recently-described specialized-cell-ensembles (e.g., capillary-venous differences in WNT signaling, arterio-venous zonation of NOTCH/DLL4, perivascular-fibroblast heterogeneity, BBB-functional subtypes). This is a tractable input for BB longevity + BBB-aging + neurodegeneration + vascular-target nomination: (a) reconstruct the molecular atlas of the human neurovascular unit at single-cell and subcellular resolution; (b) cross-reference with mouse BBB atlases (the Tabula Muris + Vanlandewijck et al. 2018 mouse-BBB atlas) to identify human-specific vascular specialization; (c) benchmark against published human AD/multi-sclerosis BBB atlases to nominate BBB-rejuvenation / vascular-aging-reversal targets (GPNMB, VCAM1, PDGFRB, FOXC1, LAMA2, COL4A1). Four-axis score: peptide 0/3, AI-agent infrastructure 3/3 (Xenium, spatial, n=15 atlas), longevity 3/3 (BBB-aging + neurodegeneration is core BB longevity lane), low-cost/ease 2/3 (n=15, H5+PARQUET+TIFF raw panel), translational fit 1/3 = 9/12. Source: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335898. ### 4. Tracker note — Biotech Infrastructure / HuggingFace AI-DD + longevity intel scan (RECYCLED entities / refreshed metrics, NO NEW entities, Biomni rotation gap re-flagged): The 2026-08-16 HuggingFace intel scan returned 9 entries — ALL 9 are RECYCLED entities from the id:65–id:69 window (no NEW HF entities surfaced today). Stable entries with refreshed download counts: longevity-db/human-muscle-aging-atlas-snRNAseq (189 downloads, 0 likes, cc-by-4.0, the Human Skeletal Muscle Aging Atlas sn/scRNA-seq dataset 1; +0 since id:68 ingestion-tag), longevity-db/mouse-muscle-aging-atlas-snRNAseq (200 downloads, 0 likes, cc-by-4.0, the Mouse Skeletal Muscle Aging Atlas sn/scRNA-seq; +1 since id:68 ingestion-tag 201 downloads), OATML-Markslab/ProteinGym_v1 (2,042 downloads, 8 likes, the ProteinGym v1 substitution + indel benchmark), OATML-Markslab/ProteinGym_v0.1 (2,910 downloads, 15 likes, the original ProteinGym v0.1, arXiv:2205.13760), tyang816/ProteinGym_v1 (48 downloads, 0 likes, the ProtSSN dataset variant), ICML2022/ProteinGym (958 downloads, 12 likes, arXiv:2205.13760), genbio-ai/ProteinGYM-DMS (815 downloads, 1 like), Jianwen/protein_ligand_cofolding_posebusters (279 downloads, 0 likes, frozen-data revision of the Autoresearch protein-ligand co-folding task on 62 PoseBusters cases — +11 since id:69 / +11 since id:68 — still pre-release / closed-beta), SharkieJones/celldega_Visium-HD_hCRC (12 downloads, 0 likes, the celldega spatial-QC benchmark on Visium-HD human colorectal cancer — +1 since id:69 / +1 since id:68). Notably ABSENT today: biomni/Biomni-R0-32B-Preview (was 3,366 cumulative downloads as of id:67 / 2,959 as of id:66 — the watcher’s HF query rotation has now dropped this entity for two consecutive scans, indicating a chronic rotation-coverage gap on the most-downloaded biomedical LLM of the past 30 days); also absent: boltz-community/boltz-2 (still pre-release), krkawzq/BiomniGEM. This is a RECYCLED entities / refreshed metrics entry plus a Biomni rotation-gap re-flag — NO NEW HuggingFace entities today, so it does NOT count toward the novelty gate but is worth tracking as a continuous signal of the BB AI-DD + longevity + biotech-infrastructure lane. Recommended BB move: (a) escalate the Biomni-R0-32B-Preview HF rotation gap to the watcher team (the watcher’s HF query rotation is dropping the most-downloaded biomedical LLM from coverage); (b) wait for boltz-2 public weights before benchmarking against AlphaFold3-Multimer + Chai-1; (c) continue to monitor longevity-db/human-muscle-aging-atlas-snRNAseq and longevity-db/mouse-muscle-aging-atlas-snRNAseq for the cross-1,000 downloads threshold (~189 → ~200 → ~250 trajectory); (d) integrate the Jianwen protein-ligand co-folding PoseBusters benchmark into the BB boltz-2 readiness pipeline once weights land. ### 5. Novelty and action gate — All three actionable signals (GSE338175 circRNA-eWAT, GSE306111 FALD-liver-CosMx, GSE335898 brain-vasculature-Xenium) are absent from the prior id:65–id:69 window at both accession and study-family level (NOVEL 3/3, passes the goal criterion of ≥2/3 NOVEL). Combined four-axis score (peptide / AI-agent infrastructure / longevity / low-cost-ease / translational-fit): circRNA-eWAT 8/12 + FALD-liver-CosMx 9/12 + brain-vasculature-Xenium 9/12 = 26/36 — the highest combined 4-axis score in the past 5 days (well above the 19–24/36 range seen in id:67–id:69) and the strongest combined-longevity-lane digest in the past week. Today is the FIRST id:65–id:70 scan where the entire score-9 ladder is fully recycled (zero fresh score-9 NOVEL), making the actionable NOVEL signals fall into the score 6–7 band — a pattern that should be tracked for potential 'thin-scan' recurrence in subsequent cycles. The longevity + adipose-browning lane is fresh for the first time in id:65–id:69 (1/3 today vs 0/3 in id:67–id:69), and the longevity + MASH / fatty-liver lane is fresh for the second time (1/3 today vs 0/3 in id:67–id:69, but complementing id:65 HAND2-BMP5-SMAD1/5/9 hepatic-stellate + GSE330687 NSCLC-metabolism cross-reference). Recommended BB move: (a) ingest GSE338175 TXT raw files to reconstruct the circRNA-host-gene regulatory network in eWAT and benchmark against the existing BB longevity + metabolic-disease reference panel (id:69 GSE343103 FOXA1-TRIM28 prostate-cancer, id:69 GSE305335 TKT-super-enhancer prostate-cancer, id:66 GSE342353 O-GlcNAc antibody) for a unified longevity + adipose-browning + metabolic-disease brief; (b) ingest GSE306111 CSV raw files to reconstruct the human FALD CosMx spatial atlas and benchmark against the existing BB MASH / fibrosis / liver reference panel (id:65 GSE341139+GSE341616 HAND2-BMP5-SMAD1/5/9 hepatic-stellate, id:65 GSE330687 PD-L1 NSCLC-metabolism cross-reference) for BB MASH / anti-fibrotic briefs; (c) ingest GSE335898 H5+PARQUET+TIFF raw files to reconstruct the human-brain-vasculature Xenium atlas and benchmark against the existing BB neuroscience / neurodegeneration reference panel (this is the FIRST dedicated brain-vasculature / BBB atlas in the digest — a fresh entry for BB longevity + BBB-aging + vascular-target nomination); (d) escalate the Biomni-R0-32B-Preview HF rotation gap to the watcher team. Do not treat the small-n atlases (n=6 mouse eWAT, n=5 human FALD, n=15 brain vasculature) as definitive clinical evidence — these are hypothesis-generation inputs that should be benchmarked against existing reference atlases before commissioning any wet-lab follow-up. --- ## 💼 Next Steps - **[Open GSE338175 in GEO (circRNA BRAKE on eWAT, n=6 RNA-seq)](https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338175)** - **[Open GSE306111 in GEO (human FALD + normal liver CosMx spatial, n=5)](https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE306111)** - **[Open GSE335898 in GEO (human brain vasculature Xenium spatial atlas, n=15)](https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335898)** - **[Track longevity-db/human-muscle-aging-atlas-snRNAseq on HuggingFace](https://huggingface.co/datasets/longevity-db/human-muscle-aging-atlas-snRNAseq)** - **[Track Jianwen/protein_ligand_cofolding_posebusters on HuggingFace](https://huggingface.co/datasets/Jianwen/protein_ligand_cofolding_posebusters)** - **[Track SharkieJones/celldega_Visium-HD_hCRC on HuggingFace](https://huggingface.co/datasets/SharkieJones/celldega_Visium-HD_hCRC)** - **[Request longevity + adipose-browning + MASH + BBB-aging brief](https://brownbio.tech/services/ai-drug-discovery#brief)** --- ## 📡 Provenance - **Source:** research-watcher (PubMed/GEO scan, 27 query families × Brown Biotech pipelines) - **Pipeline:** [`brown-biotech-daily-tech-digest`](https://github.com/ohbryt/brown-biotech-platform) — 06:00 KST cron - **Generated:** 2026-08-16 06:12 KST - **Repo:** `ohbryt/brown-biotech-platform` --- _Auto-published by `brown_biotech_research_digest_publisher.py` · [brownbio.tech](https://brownbio.tech) · Decision-ready research, daily._
Brown Biotech Research Digest — 2026-08-16
PubMed/GEO scan · research-watcher · 06:00 KST