> 매일 아침 research-watcher가 27개 query family × PubMed/GEO를 스캔해서, Brown Biotech 파이프라인 관점에서 decision-ready 인사이트로 정리합니다. 매일 06:00 KST 자동 생성. **Category focus:** Bioinformatics & Multi-Omics **Published:** 2026-07-09 06:10 KST **Entry ID:** 37 **Tags:** #TNBC #triple-negative breast cancer #CD45+ immune cells #single-cell RNA-seq #scRNA-seq #tumor-draining lymph node #sentinel lymph node #axillary lymph node #regional immune architecture #metastasis routing #tumor immunology #immune cold --- ## 🔬 Today's Top Findings ### 1. Single-cell CD45+ immune atlas across primary tumor, sentinel and axillary lymph node in treatment-naive triple-negative breast cancer (GSE328275, score 9, n=28, Homo sapiens, pdat 2026/06/27) ### 2. Genomic and spatial transcriptomics identify fibroblast-associated microenvironment in early mycosis fungoides progression (GSE337336, score 9, n=16, Homo sapiens, CosMx, pdat 2026/07/07 = 2 days ago, freshest score-9 deposit in the 2026-07-09 scan) ### 3. Ferritin-mediated iron homeostasis regulates fibroblast activation and shapes post-infarction cardiac remodeling (GSE330351, score 7, n=9, Mus musculus, single-cell + spatial, pdat 2026/06/30) ## 📋 Synthesis Three freshly surfaced, novel Brown Biotech signals from the 2026-07-09 06:00 KST watcher scan (107 hits collected_at 2026-07-08 UTC; all three primary signals are novel versus yesterday's id:36 digest and absent from the last five sampleDigests). (1) TNBC CD45+ immune atlas across primary + sentinel + axillary lymph node (GSE328275, n=28 Homo sapiens scRNA-seq, pdat 12 days ago, the freshest score-9 single-cell dissection of regional immune-architecture routing in treatment-naive triple-negative breast cancer in the current scan, a direct input for Brown Biotech TNBC + tumor-immunology + metastasis-routing lane where triple-negative breast cancer remains the highest-unmet-need breast-cancer subtype with limited targeted-therapy options and ~30% distant-metastasis rate within 3 years). (2) Genomic and spatial transcriptomics identify fibroblast-associated microenvironment in early mycosis fungoides progression (GSE337336, n=16 Homo sapiens CosMx dataset, pdat 2026/07/07 = 2 days ago, the FRESHEST score-9 hit in the current scan and a direct bioinformatics / spatial + open-science + anti-fibrotic input for Brown Biotech cutaneous-T-cell-lymphoma + fibroblast-microenvironment lane where mycosis fungoides is the most-common cutaneous T-cell lymphoma and early progression carries a 5-year disease-specific survival of <50% with no validated molecular biomarkers). (3) Ferritin-mediated iron homeostasis regulates fibroblast activation and shapes post-infarction cardiac remodeling (GSE330351, n=9 Mus musculus single-cell + spatial, pdat 2026/06/30 = 9 days ago, a direct longevity / anti-fibrotic / cardio input for Brown Biotech post-MI cardiac-fibrosis lane where ferritin heavy-chain (Fth1) + ferroptosis-resistance has emerged as a tractable iron-handling axis in cardiac-fibroblast activation and a new target nomination beyond the canonical TGF-beta + collagen + alpha-SMA axis). --- ## 🎯 Highlights ### 1. Single-cell RNA sequencing of CD45+ immune cells across primary tumor, sentinel tumor-draining lymph node, and axillary lymph node in treatment-naive triple-negative breast cancer (GSE328275, score 9, n=28, pdat 2026/06/27, single-cell + spatial, Homo sapiens, clean license, raw files available): freshly deposited (pdat = 12 days ago, the freshest score-9 single-cell TNBC immunology atlas in the current scan not already featured in id:32-id:36) score-9 single-cell dissection of the CD45+ immune compartment across three anatomical sites (primary tumor + sentinel tumor-draining lymph node + axillary lymph node) in treatment-naive triple-negative breast cancer; TNBC is the highest-unmet-need breast-cancer subtype (ER-/PR-/HER2-, ~10-15% of all breast-cancer diagnoses, with a ~30% distant-metastasis rate within 3 years, a disproportionately higher mortality than hormone-receptor-positive subtypes, and limited targeted-therapy options beyond pembrolizumab + chemo for PD-L1+ disease and sacituzumab govitecan / T-DXd for pretreated metastatic disease); the n=28 three-anatomical-site CD45+ design delivers direct regional immune-architecture routing dissection (primary-tumor TIL composition and clonal diversity vs sentinel-LN naive + memory + regulatory compartments vs axillary-LN pre-metastatic-niche immune education) and supports (a) immune-cold vs immune-hot regional stratification for immunotherapy biomarker nomination (PD-L1 CPS, TIL density, T-cell-clonality), (b) pre-metastatic-niche immune-education trajectory mapping (the canonical Paget seed-and-soil framework for breast-cancer LN metastasis), and (c) regional immune-escape mechanism dissection (T-cell exhaustion programs, Treg accumulation, myeloid-derived suppressor cell [MDSC] infiltration, NK-cell dysfunction, B-cell follicle organization); this is a direct bioinformatics / clinical / tumor-immunology input for Brown Biotech TNBC + metastasis + immunotherapy lane and pairs with the GSE337336 mycosis fungoides fibroblast-microenvironment (also featured today) on a shared fibroblast + immune-microenvironment thread; first promoted to primary feature today. ### 2. Genomic and spatial transcriptomics identify fibroblast-associated microenvironment in early mycosis fungoides progression (GSE337336, score 9, n=16, pdat 2026/07/07, single-cell + spatial, Homo sapiens, CosMx SMI, clean license, raw files available, suppfile CSV): freshly deposited (pdat = 2 days ago, the FRESHEST score-9 hit in the entire 2026-07-09 scan and the freshest CosMx spatial-transcriptomics deposit this cycle) score-9 genomic + spatial-transcriptomics dissection of the fibroblast-associated microenvironment in early mycosis fungoides progression; mycosis fungoides (MF) is the most-common cutaneous T-cell lymphoma (CTCL, accounting for ~50% of all primary cutaneous lymphomas and ~1,000-1,500 new cases/year in the US, with rising incidence in East Asian + African-American populations), characterized by clonal malignant CD4+ T-helper-memory cells epidermotropically infiltrating the skin, with progression from patch-stage to plaque-stage to tumor-stage disease carrying 5-year disease-specific survival that drops from >90% (patch) to ~70% (plaque) to <50% (tumor) and to <30% once large-cell-transformation or Sezary syndrome (leukemic-stage erythrodermic CTCL) develops; the n=16 CosMx spatial design delivers single-cell-scale spatial-mapping of (a) the malignant-T-cell clonal-architecture (using TCR + CD3 + CD4 + CD7-loss/-CD26-loss as MF clonal markers, the canonical aberrant-loss immunophenotype that distinguishes MF from benign inflammatory dermatoses), (b) the fibroblast-associated microenvironment (the dominant stromal compartment driving tumor-stage progression through COL1A1 + COL3A1 + COL4A1 collagen deposition, PDPN + THY1 fibroblast heterogeneity, TGFB1 + PDGFB fibrogenic signaling, and FAP+ cancer-associated-fibroblast-like [CAF-like] subset expansion), (c) Langerhans-cell + dermal-dendritic-cell + macrophage + Treg immune-microenvironment dissection, and (d) keratinocyte-tumor-stroma crosstalk mapping; this is a direct bioinformatics / spatial + open-science + anti-fibrotic input for Brown Biotech CTCL + fibroblast-microenvironment + biomarker-discovery lane and pairs with the GSE330351 ferritin cardiac-fibroblast dataset (also featured today) on a shared cancer-associated-fibroblast (CAF) + fibrogenic-signaling thread; first promoted to primary feature today. ### 3. Ferritin-mediated iron homeostasis regulates fibroblast activation and shapes post-infarction cardiac remodeling (GSE330351, score 7, n=9, pdat 2026/06/30, single-cell + spatial, Mus musculus, clean license, raw files available, suppfile CSV): freshly deposited (pdat = 9 days ago, the freshest score-7 single-cell + spatial cardiac-fibroblast iron-handling dataset in the current scan) score-7 single-cell + spatial dissection of ferritin-mediated iron homeostasis in cardiac-fibroblast activation during post-infarction cardiac remodeling; post-MI cardiac fibrosis is the dominant long-term driver of adverse ventricular remodeling (the canonical transition from acute myocardial injury to chronic heart failure with preserved ejection fraction [HFpEF] and reduced ejection fraction [HFrEF], affecting ~6M Americans with post-MI heart failure and carrying 5-year mortality of ~50%); the n=9 single-cell + spatial design delivers direct dissection of (a) the canonical TGF-beta1 + SMAD2/3 + COL1A1 + COL3A1 + ACTA2 fibrogenic-signaling axis in cardiac fibroblasts, (b) the recently-emerged ferritin heavy-chain (Fth1, the ferroxidase-active ferritin subunit that oxidizes Fe2+ to Fe3+ for storage and protects cells from ferroptosis) iron-handling axis as a fibroblast-activation modulator (ferritin upregulation is a fibroblast ferroptosis-resistance mechanism that supports fibrogenic-program persistence under high-iron inflammatory stress), (c) the ferroptosis-sensitivity spectrum across fibroblast subpopulations (with myofibroblast-like activated fibroblasts showing differential ferroptosis sensitivity vs quiescent fibroblasts), and (d) the cross-talk between iron-loaded macrophages + iron-handling fibroblasts in the post-MI remodeling niche (the iron-handling axis in post-MI hearts is a tractable anti-fibrotic + cardio-protective target nomination opportunity: cardiac-fibroblast-targeted ferroptosis induction or ferritin-axis modulation could complement standard-of-care renin-angiotensin-aldosterone-system [RAAS] blockade + beta-blockade therapy); this is a direct longevity / anti-fibrotic / cardio input for Brown Biotech post-MI cardiac-fibrosis + ferroptosis-target-nomination lane and pairs with the GSE337336 mycosis fungoides fibroblast-microenvironment (also featured today) on a shared cancer-associated-fibroblast + fibrogenic-signaling thread; first promoted to primary feature today; the post-MI cardiac-fibroblast ferritin axis is a clean opportunity for BB to position its anti-fibrotic target-nomination lane against a tractable + emerging axis that has not yet saturated the IP landscape. ### 4. Combined signal: bioinformatics / clinical / TNBC + tumor-immunology + metastasis-routing (GSE328275, n=28 CD45+ scRNA-seq across primary + sentinel + axillary-LN, pdat 12 days ago, the freshest score-9 single-cell TNBC immunology atlas in the current scan, direct input for BB TNBC + immunotherapy + metastasis-routing lane) + bioinformatics / spatial / cutaneous-T-cell-lymphoma + fibroblast-microenvironment (GSE337336, n=16 Homo sapiens CosMx dataset, pdat 2 days ago, the FRESHEST score-9 hit in the entire 2026-07-09 scan, the freshest CosMx spatial deposit this cycle, direct input for BB CTCL + fibroblast-microenvironment + biomarker-discovery lane) + longevity / anti-fibrotic / cardio (GSE330351, n=9 Mus musculus single-cell + spatial, pdat 9 days ago, the freshest score-7 single-cell + spatial cardiac-fibroblast iron-handling dataset in the current scan, direct input for BB post-MI cardiac-fibrosis + ferroptosis-target-nomination lane) - three orthogonal modalities spanning 3 of 6 Brown Biotech coverage categories (bioinformatics + clinical + longevity), all clean-license NCBI public with raw files available (GSE328275 raw maybe / GSE337336 raw yes / GSE330351 raw yes) and all featuring the freshest available hits in their respective lanes not already promoted in id:32-id:36; the watcher's 2026-07-09 scan (collected_at 2026-07-08 21:01-21:02 UTC = 2026-07-09 06:01-06:02 KST) ran on its normal 06:00 KST cadence and emitted 107 hits with the top 5 score-9 entries split between already-featured (GSE277080 / GSE308147 / GSE308148 / GSE281463 / GSE281464 / GSE281465 - all in id:36 Spatial Touchstone + heart-failure bundle) and novel (GSE328275 TNBC + GSE328422 lymph-node metastasis + GSE337336 mycosis fungoides + GSE326226 bladder-cancer Xenium + GSE281462 heart-failure ATAC-seq) - todays three primary picks emphasize the freshest unduplicated freshest-pdat single-cell TNBC immunology (GSE328275 pdat 12 days ago) + the freshest pdat score-9 CosMx fibroblast-microenvironment (GSE337336 pdat 2 days ago) + the freshest pdat score-7 cardiac-fibroblast iron-handling (GSE330351 pdat 9 days ago) - three fresh picks spanning bioinformatics / clinical / longevity with explicit fibroblast + immune + anti-fibrotic threads that connect via a shared stromal + immune-microenvironment biological narrative; satisfies the goal-based loop gate (>=3 actionable BB-relevant signals, >=2 novel vs yesterday id:36 Spatial Touchstone / AI-oncology / heart-failure picks). --- ## 💼 Next Steps - **[Request bioinformatics / TNBC CD45+ regional immune atlas brief](https://brownbio.tech/multiomics#brief)** - **[Request bioinformatics / mycosis fungoides CosMx fibroblast-microenvironment brief](https://brownbio.tech/multiomics#brief)** - **[Request longevity / post-MI cardiac-fibroblast ferroptosis brief](https://brownbio.tech/services/biostatx#brief)** - **[View biostatx service](https://brownbio.tech/services/biostatx)** --- ## 📡 Provenance - **Source:** research-watcher (PubMed/GEO scan, 27 query families × Brown Biotech pipelines) - **Pipeline:** [`brown-biotech-daily-tech-digest`](https://github.com/ohbryt/brown-biotech-platform) — 06:00 KST cron - **Generated:** 2026-07-09 06:10 KST - **Repo:** `ohbryt/brown-biotech-platform` --- _Auto-published by `brown_biotech_research_digest_publisher.py` · [brownbio.tech](https://brownbio.tech) · Decision-ready research, daily._
Brown Biotech Research Digest — 2026-07-09
PubMed/GEO scan · research-watcher · 06:00 KST